Development & Validation of RP-HPLC Method for the Determination of Oseltamivir Phosphate API

  • Tabassum Bano Oxford College of Pharmacy, Massorie Industrial Area, UPSIDC, MG Road, Ghaziabad., U. P., 201001, India.
  • Rupesh Dudhe Department of Pharmacy, Galgotias University, Yamuna Expressway, Greater Noida,U. P., 201306, India
  • Nitin Kumar Oxford College of Pharmacy, Massorie Industrial Area, UPSIDC, MG Road, Ghaziabad., U. P., 201001, India.
Keywords: Oseltamivir phosphate (OSP), RP-HPLC.

Abstract

A simple and rapid liquid chromatographic assay for the evaluation of potentially counterfeit oseltamivir phosphate has been developed and assessed. The present study describes the development of a new, simple, reproducible RP-HPLC method for the quantitative determination of Oseltamivir phosphate (OSP). The proposed method utilizes C-18 column 100mm x 4.6mm at 25?C with isocratic run using Methanol and Acetonitrile (50:50 v/v) at a flow rate of 1ml/min. and UV detection at 227 nm. The method was statistically validated for linearity, ruggedness, robustness, precision, and accuracy. The calibration curve was obtained in the concentration range of 5-35?g/ml with correlation coefficient 0.996. The robustness of the method has been studied by slightly varying the chromatographic conditions.

Downloads

Download data is not yet available.

References

S M Malipatil , Kishwar Jahan, S K Patil Indo-Global Journal of Pharmaceutical Sciences, 2011, Vol 1., Issue 1: Page No. 57-62.

J. Joseph-Charles a, C. Geneste a, E. Laborde-Kummera, R. Gheyouche b, H. Boudis b, J.-P. Dubost a, Journal of Pharmaceutical and Biomedical Analysis 44 (2007) 1008–1013.

World Health Organization, WHO Media Centre, Counterfeit medicines, Fact sheet No. 275, Revised February 2006, Available from http://www.who.int/mediacentre/factsheets/fs275/en/.

N. Lindegardh a,b, T.T. Hien c, J. Farrar b,d, P. Singhasivanon a, N.J. White a,b, N.P.J. Daya,b , Journal of Pharmaceutical and Biomedical Analysis 42 (2006) 430–433.

Michael D. Green, Henry Nettey, and Robert A. Wirtz, pdf

Aleksander P. Winiarski, Martin H. Infeld, Ronald Tscherne, Maria Bachynsky, Richard Rucki, and kathy Nagano–Mate J o u r n a l o f t h e Ame r i c a n Ph a rma c i s t s As s o c i a t i o n www.pha rma c i s t . com Nov/Dec 2007 • 47:6 • JAPhA • 747.

M. Espinosa Boscha, C. Bosch Ojedab, A.J. Ruiz Sánchezc, F. Sánchez Rojasb1 , July – September 2010 RJPBCS Volume 1 Issue 3 Page No. 368.

Evelyne Laborde-Kummera, Karen Gaudina, Julienne Joseph-Charles , Rachida Gheyoucheb Hakim Boudisb, Jean-Pierre Dubost , Development and validation of a rapid capillary electrophoresis method for the determination of oseltamivir phosphate in Iamiflu and generic versions, Journal of Pharmaceutical and Biomedical Analysis 50 (2009) 544–546.

Balasubramanian N, Khan A, Kona S. Stability indicating RP-HPLC method development and validation for Oseltamivir API. Chemical & Pharmaceutical Bulletin. 2008;4(56):413-417.

Published
2015-07-11
How to Cite
Bano, T., Dudhe, R., & Kumar, N. (2015). Development & Validation of RP-HPLC Method for the Determination of Oseltamivir Phosphate API. Journal of Progressive Research in Chemistry, 2(1), 69-73. Retrieved from http://scitecresearch.com/journals/index.php/jprc/article/view/193
Section
Articles