Efficient DNA Extraction Technique from Leave Tissues of Some Important Tropical Plant Species
Abstract
ABSTRACT
Extraction of DNA is very important aspect in the plant molecular biological investigations. In this study, a large number of samples such as fresh leaves obtained from different plant species Oryza sativa, Elusine korakkana, Zea mays, Azadirachta indica, Musa sapianatum, Sesbania grandiflora were taken and faster method of Lin Rong et al (2001) applied for DNA extraction that can provide huge amount of high quality DNA determined by horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60V. Purity was measured by the ratio of 260/280nm near to 1.8 by using Nanodrop ND-1000 spectrophotometer.
Downloads
References
Akshatha Venugopalan, Giridhar P, and Ravishankar, GA, (2011). Food Ethanobotanical and Diversified alication of Bixa orellana a scope for its improvement through biotechnology mediation. Indian Journal of Fundamental and Allied Life Sciences, 1(4), 9-31.
Anil Kumar, Niharika, G, Bhawsar, Poornima Badnagre, Ujjaval Panse, SR, Gayakwad, and Krishna Khasdeo, (2013a). Isolation of Agrobacterium tumefaciens from soil and Optimization of Genomic & Plasmid DNA Extraction. IJAR, 1(2), 1-4.
Anil Kumar, Akansha Gayakwad, Panse, U, Khasdeo, K, Narayanan, C, Ansari, SA, and Asha, D, Lazarus, (2013b). Optimization of DNA Extraction Methods for Some Important Forest Tree Species IJABR, 4(3), 364-371.
Arote, SR, and Yeole, PG, (2010). Pongamia pinnata L, A Comprehensive Review. International Journal of Pharmacological Tech Reearch, 2(4), 2283-2290.
Cheng, FS, Brown, SK, and Weeden, NF, (1997). DNA extraction protocol from various tissues in woody species. HortScience 32(5):921-923.
Csaikl, UM, Bastian, H, Brettschneider, R, Gauch, S, Mier, A, Schauerte, M, Scholz, F, Spierson, C, Vornam, B, and Ziegenhagen, B, (1998). Comparative analysis of different DNA extraction protocols: a fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies. Plant Molecular Biology Reporter, 16(1), 69-86.
Debjit Bhowmik, Chiranjib, Jitender Yadav, Tripathi, KK, Sampath Kumar, KP, (2010). Herbal Remedies of Azadirachta indica and its Medicinal Alication. J Chem Pharm Res, 2(1), 62-72.
Henry, RJ, Plant DNA extraction. In, Henry, R.J. (ed.) (2001). Plant Genotyping, the DNA fingerprinting of plants. CAB International, United Kingdom, 239-249.
Loomis, MD, (1974). Overcoming problems of phenolics in the isolation of plant enzymes and organelles. Methods in Enzymology, 31, 528-545.
Shepherd, M, Cross, M, Stoke, LR, Scott, LJ, and Jones, ME, (2002). High-Throughput DNA Extraction from Forest Trees. Plant Molecular Biology Reporter, 20, 425-425.
Subapriya, R, Nagini, S,. (2005). Medicinal properties of neem leaves, a review. Curr Med Chem Anti-Canc Agents, 5, 149–56.
Weising, K, Nybom, H, Wolff, K, and Meyer, W, (1995). DNA isolation and purification. In, DNA fingerprinting in plants and fungi. Press. Boca Raton, Florid, 44-59.
Lin Rong-Cheng, Zai-Song Ding, Liang-Bi Li and Ting-Yun Kung, (2001). A rapid and efficient DNA mini preparation suitable for screening transgenic plants. Plant Molecular Biology Reporter, 19, 379a-379e.
Copyright (c) 2015 Journal of Progressive Research in Biology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
JPRB is pleased to undertake the publication of your contribution to Journal of Progressive Research in Biology.
The copyright to this article is transferred to JPRB(including without limitation, the right to publish the work in whole or in part in any and all forms of media, now or hereafter known) effective if and when the article is accepted for publication thus granting JPRB all rights for the work so that both parties may be protected from the consequences of unauthorized use.
